Edwards Pirani Penning 1005 Manual Woodworkers

Contents Retrieved from Microscopy Listserver ArchivesAccepting credit cards for your businessHas never been so easy & affordable!Our specialty is establishing your merchant account!NO APPLICATION FEE!NO PROGRAMMING FEE!$9.95 PROCESSING FEEIf you already accept credit cards we can save you $$$. We offer theabsolute lowest rates in the industry!

Contents Retrieved from Microscopy Listserver ArchivesColleagues.Sorry all but it looks like I screwed up again. Over the weekendI was editing the filtering software trying to increase theSPAM/Junk Mail rejection capabilities. Well, I manaaged to stopall mail to the List which means I was 100% effectivein filtering the Junk Mail!!;-)In any event, I think things are back on track, and working again.If you tried to post anything between Oct 1 and todayyou should consider it as being permanently lost in the Ether.Just resubmit them.BTW, thanks to all those people who started sending me Emailsaying ' I haven't gotten any mail in the last few days' it startedme looking for problems. I guess it's good to know you all missyour Daily Microscopy Email-Fix. Almost sounds addictive.(Hmmm. Too bad I can't bottle it an make a few $$)Cheers.NestorYour Friendly Neighborhood SysOp.From daemon Wed Oct 4 10.

Contents Retrieved from Microscopy Listserver ArchivesI have an application for separating various size particles using ThinLayer Chromatography.Question, has anyone prepped the polyester backing, with silica gelcoating for SEM?I have a couple hypothetical protocols I could try but I thought I wouldsend this question out to the masses, to see if anyone has hadexperience examining TLC plates in the SEM (regluar SEM not an ESEM orVPSEM).Thanks in Advance,Geoff WilliamsElectron Microscope Facility SupervisorBiology DepartmentBrooks HallCentral Michigan UniversityMt. Pleasant, MI 48859Geoffrey.Lloyd.Williams-at-cmich.edu517 7 774-3462 (fax)From daemon Wed Oct 4 11. Contents Retrieved from Microscopy Listserver ArchivesA colleague on campus is having a problem with their ISI Alpha 9 SEM.They have diagnosed a failure of the high voltage power supply.Specifically, the gun side of the multi-tap power supply has beenfried.So, they are looking for the power supply for the Alpha 9 or an ISIMini SEM. If anyone has such a component (or even the whole SEM) forsale or donation, please contact me.Thank you.John B.####################################################################John J. Bozzola, Ph.D., DirectorIMAGE (Integrated Microscopy & Graphics Expertise)750 Communications Drive - MC 4402Southern Illinois UniversityCarbondale, IL 62901 U.S.A.Phone: 618-453-3730Fax: 618-453-2665Email: bozzola-at-siu.eduWeb: daemon Wed Oct 4 11. Contents Retrieved from Microscopy Listserver Archives The Chemical Technology Division of Argonne National Laboratory has an opening for a staff microscopist to perform transmission electron microscopy in support of the nuclear fuel cycle, waste management, and other programs as required. This person will also develop new processes and/or equipment and other technology to support the programs, and interface with sponsors to maintain existing support and develop new initiatives.

This position requires a Ph.D. In chemistry, materials science, or related discipline and 2-5 years of experience (or equivalent). For further information or to send resume, contact David Chamberlain by electronic mail: chamberlain-at-cmt.anl.gov.From daemon Wed Oct 4 12. Contents Retrieved from Microscopy Listserver ArchivesDear Listners:Any comments on which would make a better detector, Ge or Si? Only PGTsells Ge detectors and I'm aware of the decrease in resolution with Gedetector.

Could someone lists or details the benefits of having a Gedetector as oppose to a Si one.SincerelyN.V.VoN.V.Vo, Ph.D.Intermagnetics General Corporation450 Duane AvenueSchenectady, New York 12304Phone: 518 346 1414 x 3107Fax: 518 346 6080E-mail: nvo-at-igc.comwww.igc.comFrom daemon Wed Oct 4 13. Contents Retrieved from Microscopy Listserver ArchivesSimonI am not a diatom expert, but I know someone who is. Put yourquestion to Professor David Mann at the Royal Botanic GardenEdinburgh. He eats, sleeps and breathes diatoms (maybe Iexaggerate a little!). His email address isd.mann-at-rbge.org.ukgood huntingChris Jeffree From: 'swoodland-at-madasafish.com'-at-sparc5.microscopy.comDate sent: Wed, 4 Oct 2000 10:04:39 -0500To: Microscopy-at-sparc5.microscopy.comFaculty PositionDepartment of Materials Science and EngineeringLEHIGH UNIVERSITYLehigh seeks to fill a tenure-track position, at the level of associateor assistant professor, in Materials Science and Engineering. Researchinterests in any area of materials will be considered but the personselected will have a major commitment to the use of electron microscopy.The Materials Department at Lehigh has research strengths in areas ofceramics, metals, polymers and electronic materials.

Details can befound from the faculty pages at the departmental web site:It will be considered anadvantage if the research interests of the applicant are related toexisting research interests in such a way that collaborative work ispromoted. Lehigh runs an outstanding electron microscopy facility.Favorable consideration will be given to candidates whose research willinvolve substantial use of electron microscopy, especially when themicroscopy is innovative rather than routine.

Lehigh is committed torecruiting, retaining and tenuring women and members of minority groups.Please submit an application by December 15, 2000, to Sharon Coe,Department of Materials Science and Engineering, Lehigh University, 5East Packer Avenue, Bethlehem PA, USA (slc6-at-lehigh.edu). Todiscuss the post contact Alwyn Eades (jae5-at-lehigh.edu) at the same address.Dr. ZaluzecMaterials Science DivisionBuilding 212Argonne National Lab9700 S. Cass AveArgonne, Illinois 60439 USATel: 630-252-7901, Fax: 630-252-4798Email: Zaluzec-at-aaem.amc.anl.govTPMLab: box said 'This program requires Win 95/98/NT or better.' So I boughta G3 MacFrom daemon Wed Oct 4 13.

Contents Retrieved from Microscopy Listserver ArchivesThe Minnesota Microscopy Society will be holding it's annual Fall Kick-Off eventon Thursday, October 12, 2000This will follow the traditional format of a dinner followed by a talk, whichthis year is:'Nature's Jewels and Scientific Tools: Confessions of a Career Diatomist'by Mark B. Edlund from the Science museum of Minnesota.Abstract:Diatoms are single-celled or colonial micro-organisms that possess a cell wallof ornamented opaline silica (biologically-produced glass) and are commonlyreferred to as the golden-brown algae. It is the wall ornamentation and shapethat are used to identify the nearly 25,000 species of living diatoms. Diatomswere 'discovered' within the first hundred years of microscopy; Leeuwenhoekhimself may be credited with one of the earliest illustrations (1703). Sincethat time, diatoms have been the jewels, the postage stamps, and the tools ofamateur and professional diatomists. Microscopical societies burgeoned in thelatter half of the 19th century with members actively exchanging diatomaceousmaterial, slides, and methods and discussing publications and exsicattae.Diatoms figured strongly in the development of light microscopy as testorganisms for resolving power; even today, many microscopists have anAmphipleura pellucida slide in the drawer. Transmission electron microscopyrevealed much-needed knowledge on the cytology and mechanism of cell wallformation in diatoms, but it has been the scanning electron microscope that hastruly changed the field.

Ultrastructural details seen in the SEM coupled with'rediscovery' of beautiful cytological work done in the late 1800's and early1900's radically changed our modern understanding of diatom systematics. Lastly,diatoms have proved to be excellent indicators of water quality andenvironmental change. Whereas descriptive and biodiversity studies are stillcommon, applied methods now dominate the current scientific efforts.Speaker: Mark B. Edlund, Research ScientistScience Museum of MinnesotaMark Edlund has a PhD in Natural Resources from the University of Michigan, andhas been specializing in diatoms for the last ten years. Besides field work inthe United States, he has done work at Lake Baikal in Siberia, and recently inMongolia.Program:5:30-6:00 PM Social hour6:00-7:00 PM Dinner7:00-7:15 PM Business meeting7:15-8:15 PM SpeakerDuring the social hour, wine, cheese and crackers will be served.The cost of the meal is $20 per person.Location:Cherrywood Room, 2nd Floor, St. Paul Students Cente,r St Paul Campus, Universityof Minnesota, 2017 Buford Ave., St. PaulMake Your Reservations TodayAn accurate head count for the dinner is an absolute necessity.

Contact StuartMcKernan by Friday, October 6, to make your reservation (612-624-6009, orstuartm-at-tc.umn.edu).Stuart McKernan stuartm-at-tc.umn.eduOffice:(612) 626-7594IT Characterization Facility, University of Minnesota Desk:(612) 6 Union Street S. E., Minneapolis, MN 55455 NEW- Fax:(612) 625-5368From daemon Wed Oct 4 14. Contents Retrieved from Microscopy Listserver ArchivesI have not tried this source, but have heard:You might try a swimming pool supply store. Look for Diatomaceous Earthfilter media. As to preparation.

Techniques will depend on the type ofmicroscopy employed. If using a high vacuum electron microscope, anelectrically conductive film will need to be applied to the diatoms whichhave been (typically) sprinkled onto double faced tape. The tape will nothave to be conductive (if it is - so much the better) if the whole mount isconductively coated.Woody WhiteMcDermott Technology, Inc.daemon Wed Oct 4 14. Contents Retrieved from Microscopy Listserver ArchivesIs there a problem with the server? I haven't received any messages in the last couple of days.

I checked with our server and everything seems to be O.K.Did my address get taken off by accident? In case it was my address is: prutledge-at-ars.usda.govThanks,Phillip RutledgeUSDA/ARSAquatic Animal Research Lab.151 Dixon DriveChestertown, MD 21620voice: 410.778.4136 or 2120fax: 410.778.4399e-mail: prutledge-at-ars.usda.govFrom daemon Wed Oct 4 14.

Contents Retrieved from Microscopy Listserver ArchivesI would try the two following companies as possible sources of fineplatinum wire:GoodFellow, P.O. Box 628, Doylkestown, PA FAX: 1-800-283-2020Refining Systems Inc., P. Box 72446, Las Vegas, NV 89170FAX: 702-368-0933Both sell high purity and specialty metals in a variety of sizes and shapes.Good luck,WCBWilbur C.

Bigelow, Prof. EmeritusMaterials Sci. & Engr., University of MichiganAnn Arbor, MI e-mail: bigelow-at-umich.edu;Fx:734-763-4788; Ph:734-662-5237From daemon Wed Oct 4 14. Contents Retrieved from Microscopy Listserver ArchivesAt 10:04 AM -0500 10/4/00,'swoodland-at-madasafish.com'-at-sparc5.microscopy.com wrote: - The Microscopy ListServer - Sponsor: The Microscopy Society of AmericaHave you tried Carolina Biological? They have all kinds of pre-mountedslides for sale.Leona Cohen-Gould, M.S.Sr.

Staff AssociateDirector, Electron Microscopy Core FacilityManager, Optical Microscopy Core FacilityJoan & Sanford I. Weill Medical Collegeof Cornell Universityvoice (212)746-6146fax (212)746-8175From daemon Wed Oct 4 16. Contents Retrieved from Microscopy Listserver ArchivesAnyone know of a easy, simple way to produce nice powder diffraction samples for illustrating the powder diffraction method for EM students? We use the International Centre for Diffraction Data For student identification of unknown samples. I have tried several methods that usually result in non-electron transparent samples (our TEM KV max is only 120). Any ideas?Bill CarmichaelElectron Microscopy FacultyMadison Area Technical College3550 Anderson St.Madison, WI 53704608-243-4309wcarmichael-at-madison.tec.wi.usdaemon Wed Oct 4 16.

Contents Retrieved from Microscopy Listserver ArchivesMacalester College (St. Paul, Minnesota) has a job opening which involveslooking for someone with a microscopy background (as well as electronicsskills). The job description is posted at:description is quite brief, but Macalester has both a Zeiss EM109 TEMand a Zeiss DSM960A SEM.

Maintenance and instruction for students/facultyon these machines will be one aspect of this position.Regards,Jeff TholeJeff Thole - Geology Laboratory Supervisor and InstructorGeology Department, Macalester College1600 Grand Avenue, St. Paul, MN 55105(ph. 651-696-6426, fax. X6122, email thole-at-macalester.edu)From daemon Wed Oct 4 17. Contents Retrieved from Microscopy Listserver ArchivesTake an oxide powder, grind it up with mortar and pestle and take a carboncoated grid and swipe it across the fines. You can also use two glassslides to grind the powder up.You can evaporate a number of materials onto a carbon coated grid or onto acleaved NaCl sample and then float that off on water onto a grid.You could smoke a Mo wire in air to provide crystals if you leave it in thesmoke long enough, you will probably have enough to cover the grid. Heatthe wire across the terminals of an evaporator or heat with a torch togenerate the white smoke.

You could also burn some Mg and get MgO crystals.If you don't have sufficient particles in a single diffraction pattern togive you rings, take multiple exposures from different areas on the samepiece of film.Incidentally, the MoO3 samples will provide you with a rotation calibrationsample.-ScottScott D. Walck, Ph.D.PPG Industries, Inc.Glass Technology CenterGuys Run Rd. Contents Retrieved from Microscopy Listserver ArchivesI'm trying to solve a high resolution noise problem with a XL-30Phillips FESEM. The room that it is in was recently checked and was ok,accept for some acoustic noise. To fix the noise I put up 3 inch soundproofing foam and quieter ducting for the air condition. The noiseshowed up in an image by a streak about 5 jaggies.

Afterputting up the foam the noise was reduced, but it is still there. Thenoise is the same for 4mm working distance or a 10mm working distance.Any one had a similar problem or have any advice?Thanks,Ben############O -Ben Craft-####//#### /'### From daemon Wed Oct 4 22. Contents Retrieved from Microscopy Listserver ArchivesGary,There's a.lot. of difference. About that between a Redwood and anElephant. And as distinctive.Diatoms are about as good a plant as can be found among theprotistans. They look like more-or-less fancy silicate pillboxes withlots of little holes.Radiolaria are amoeboid beasts with a complicated silicate internal'skeleton'.

They tend to be nested, holey spheres, usually with amore-or-less complex profusion of spikes, needles, and the like.Crude descriptions, but brief. The best way to see the difference isto get a copy of Ernst Haeckel's book published by Dover - 'ArtsForms in Nature'. Beautiful drawings of many different organisms,including Radiolaria and Diatoms.Phil What is the difference between diatoms and radiolaria (Actinopods)? They 'look' the same to me. gary g.-.

Edwards Pirani Penning 1005 Manual Woodworkers Tools

Be famous! Send a Tech Tip or article to Microscopy Today!.Philip OshelTechnical Editor, Microscopy TodayP.O. Box 620068Middleton, WI Voice: days (608) 263-4162, evening (608) 833-2885Fax (608) 836-1969 (please make sure my name is on any fax)Address for UPS, FedEx, or other couriers:Dept. Of Animal Health and Biomedical SciencesUniversity of Wisconsin1656 Linden DriveMadison, WI fax: (608) 262-7420 (dept. Fax)peoshel-at-facstaff.wisc.eduFrom daemon Wed Oct 4 22.

Contents Retrieved from Microscopy Listserver ArchivesDear listers,What certified (NIST traceable?) TEM mag. Calibration samples are availablefor 20K to over 100K range? Diffraction grating replica sample will notprovide sufficient accuracy- too few grating periods 'fit' in the image athigher magnifications.Vitaly FeingoldScientific Instruments and Applications2773 Heath Lane, Duluth GA 30096(770)232-7785 ph.(770)232-1791 faxThis message is made of 100% recycled electrons.From daemon Wed Oct 4 23. Contents Retrieved from Microscopy Listserver ArchivesSimon:Try Wards Natural History Establishment in upstate New York.

Contents Retrieved from Microscopy Listserver ArchivesDear Listers!We have the problems with our JEM-4000EX: the ion pump SIP-1 is not OK!The possible causes are:1/- leak in vacuum system (after bake out of the SIP);2/-some contamination into SIP (this SIP was go OK more 40,000 hour);3/-???Does anyone know how to make testing of this situation?How do to demount the pump, what order of actions?I am not currently part of the list, so please mail to me directly.-Best regards,Anton Gutakovskii mailto:gut-at-thermo.isp.nsc.ruFrom daemon Thu Oct 5 03. Contents Retrieved from Microscopy Listserver ArchivesYes,mounting the Coolpix 990 to any photomicroscope is absolutelysimple via the usual C-mount connection. For the coolpix you haveto buy at NIKON the microscope adapter ( here in germany thisadapter is around 1000.- DM or approx. Contents Retrieved from Microscopy Listserver Archives28th SCOTTISH MICROSCOPY SYMPOSIUM15 November 2000, Moredun Institute, Edinburgh.The annual symposium is supported by all area groups in Scotland.The programme for this meeting is comprised of the following threeinvited talks together with seven contributed talks on a widerange of microscopy with general appeal and a poster display.As in recent years, a comprehensive trade exhibition by leading companieswill be present.

The Trade Exhibition, Poster Display and Cateringwill all be together in one central area of this excellent venue. ProgrammeMulti-photon microscopy. Professor Alison Gurney, Dept.ofPhysiology and Pharmacology, University of Strathclyde.Ultrastructure eggshell quality assessment: a way forward forcaptive breeding programmes. Avril Edmond, Dept.Veterinary Anatomy, Glasgow.Non-invasive imaging of plant tissues using the confocal laserscanning microscope (CLSM) Dr.

Karl Oparka, Unit of Cell Biology, SCRI,Dundee.Functional dynamics of plasmodesmata in sink/source transitionleaves. Alison Roberts, Unit of Cell Biology, SCRI, DundeeCOSMIC - a new interdisciplinary facility for advancedspectroscopy, micromanipulation and imaging. Professor WilsonPoon, Dept. Of Physics and Astronomy, University of Edinburgh.Imaging intracellular location of psychoactive drugsusing fluorescence microscopy. Richard Horobin, IBLS, Glasgow.Scanning infra-red microscopy.

Alison Coats, Dept. Chemistry,Aberdeen University.Antigen retrieval allows improved visualisation of V-ATPaseimmuno-reactivity. Susan Lindsay, Caledonian University, Glasgow.Techniques in Immunocytochemistry.

Peter Jackson, ResearchHistology Unit, Dept. Of Histopathology and Molecular Pathology,Leeds General Infirmary. Talk sponsored by DACO Ltd.See web site for abstracts from previous meetingsMackenzieElectron Microscope unitDept ZoologyUniversity of AberdeenTillydrone avenueAberdeenAB24 2TZ-Tel 47Fax 96email k.s.mackenzie-at-abdn.ac.ukFrom daemon Thu Oct 5 04. Contents Retrieved from Microscopy Listserver ArchivesI hope this isn't seen as an overt advert on the list server, I just want tolet people know that this new book is available and hope it may be ofgeneral interest. Rest assured that I and my fellow co-authors get verylittle of the sales proceeds!A.Patrick GunningIFR NorwichATOMIC FORCE MICROSCOPY FOR BIOLOGISTSby V J Morris, A R Kirby & A P GunningAtomic force microscopy (AFM) is part of a range of emerging microscopicmethods for biologists which offer the magnification range of both the lightand electron microscope, but allow imaging under the 'natural' conditionsusually associated with the light microscope. To biologists AFM offers theprospect of high resolution images of biological material, images ofmolecules and their interactions even under physiological conditions, andthe study of molecular processes in living systems.

This book provides arealistic appreciation of the advantages and limitations of the techniqueand the present and future potential for improving the understanding ofbiological systems.Contents:ApparatusBasic PrinciplesMacromoleculesInterfacial SystemsOrdered MacromoleculesCells, Tissue and BiomineralsOther Probe MicroscopesReadership: Undergraduates, postgraduates and researchers in biophysics.352pp Pub. Date: Dec 1999 Imperial College PressISBN 1-86094-199-0 US$51 / £32From daemon Thu Oct 5 05. Contents Retrieved from Microscopy Listserver ArchivesHi Peter,I think one can use the 'self timer' function of the Coolpix 990 toexpose an image without touching the camera body at the time of theexposure.I can think of no engineering reason why the cable release would need tobe expensive when it becomes available. I'll bet any electronicstinkerer could make one for $30.I'm quite happy with my Cool-Pix 990. If only I could remember 10% ofits function modes and how to access them.Bart CannonCannon MicroprobeSeattleFrom daemon Thu Oct 5 07. Contents Retrieved from Microscopy Listserver ArchivesHigh-purity Germanium detectors provide.better.

resolution thanSi(Li) detectors, and also have lower low-keV background. Thehigher atomic number of Ge means they have greater x-raystopping power and therefore higher detection efficiency for the Klines of the heavier elements, and they have therefore been thoughtof as primarily useful at higher beam accelerating voltages forheavy element analysis. However, this may only be one of theiradvantages. The better resolution and lower background they offerat low keV suggests they would also be good, and perhapspreferable to Si for light element detection and peak discriminationwhich could mean more options for low-kV excitation in Fieldemission SEMs.What are the flaws in this argument?It would be interesting to hear comments on the above from anyonewho has direct knowledge and experience of both types ofdetectors.BTW besides PGT, both Gresham and Oxford sell Ge detectors. Iam not aware of any othersChris From: 'NVo-at-IGC.com'-at-sparc5.microscopy.comTo: Microscopy-at-sparc5.microscopy.comDear Listers:I am trying to find an enlarger and digital image system to print theconventional and HREM negatives. What kind of enlarger and digital imagesystem are better? Prices?Thanks a lot!Jinguo WangFrom daemon Thu Oct 5 08.

Contents Retrieved from Microscopy Listserver Archives I'm looking to replace my two year old Polaroid Digital Microscope camera (SCSI interface with color or black and white, 1600x1200 pixels). The chip has failed and I don't want to buy another Polaroid camera. I do primarily black and white photography from a Zeiss optical microscope for materials engineering applications. I need for the camera to save in tiff format without changing the dimensions of the objects. I don't need low light applications. Any suggestions?

Thanks, Robin GriffinFrom daemon Thu Oct 5 08. Contents Retrieved from Microscopy Listserver ArchivesBill,We have found a simple method for preparing drug particles for electrondiffraction. We suspend particles in a solvent by sonication for 2-3 secondsand then let the suspension settle for 10-20 seconds. The largest, heavyparticles will settle first leaving the thin, light ones in suspension. Thentake a drop (20 ul) from the top of the solution, place it on a carbon filmedgrid and allow it to dry. The result is a distribution of particles layingflat accross the grid. You need a solvent in which the particles are notsoluble.

We typically use hexane.Joe NeillyAbbott LaboratoriesD-45M, AP31200 Abbott Park Rd.Abbott Park, IL voice: (847)-938-5024fax: (847)-938-5027e-mail: joe.neilly-at-abbott.comFrom daemon Thu Oct 5 08. Contents Retrieved from Microscopy Listserver ArchivesDear colleagues:I have two questions on TEM of DNA and protein complex:1. Which method is the best, or most suitable,to study such samples?Direct spray to the mica sheet and subsequent metal rotary shadowing, orcytochrome c spreading with metal coating examined by TEM, or atomic forcemicroscopy?2.

Is there any computer software which is designed specifically for analyzethese type of images?Any comments will be greatly appreciated.YuhuiYuhui Xu, MD,PhDEM Core, NHLBI, NIHFrom daemon Thu Oct 5 08. Contents Retrieved from Microscopy Listserver ArchivesThis is a bit off the topic of microscopy, but most of us on thisserver do work in facilities that have XRD units. If anyone is selling,or knows of someone who is selling their XRD, please contact me. I am alsocurious if anyone has had any experience with the new desk tops likeRigaku has. I plan on demoing one in the future. At $50k-$60k, they arevery cost efficient compared to standard XRD units, but I am wary of theirperformance as an analytical instrument. Lou SolebelloFrom daemon Thu Oct 5 08.

Contents Retrieved from Microscopy Listserver Archiveshi all-i've installed a 35 and have some issues with a saw-tooth distortion in thescan. It stays constant regardless of KV, but the number of saw-tooths(teeth?) in a frame increases with slower scan rates. I suspectedvibration so i mounted the roughing pumps on a big foam block.nochange. I also cycled off the chiller unit with no change, so its probablynot mechanical vibrations, unless there is another vibration causingcomponent i'm overlooking.the distortion is also visible in the data field alphanumerics (they shiftand shimmy a little) with the beam turned off, so i don't suspect a HVinstability problem.can i logically deduce that there is an electrical field leakage that ismessing up the scan? Or a ground loop?

What about cable placement? Or aproblem with the 100V variac (its within 3' of the column).right now the column is a bit dirty and needs cleaning.i've never seen,for example, dirty apertures cause this type of distortion though.any suggestions would be appreciated!brian-Brian McIntyreElectron Microscopy Lab- River CampusUniv of RochesterRochester, NY 14627716-275-3058/4875From daemon Thu Oct 5 09. Contents Retrieved from Microscopy Listserver ArchivesEarl,We distributed the Polaron coaters for years.

We can assist in spareparts and perhaps service.John Arnott-LADD RESEARCH131 Dorset LaneWilliston, VT 05495 USAweb site 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)FAX 1-802-878-8074e-mail sales-at-laddresearch.comMicroscope Supplies Since 1955Earl Weltmer wrote: Hi all, I have a BioRad SC500 coater. Can anyone give me the local US rep for service? Thank You, Earl WeltmerFrom daemon Thu Oct 5 09. Contents Retrieved from Microscopy Listserver ArchivesHello Vitaly,There currently is no NIST-traceable standard for high-magnificationTEM on the market. The next best thing is the MAG.I.CAL TEM calibrationsample, which is internally calibrated against the lattice spacing ofsilicon, a fundamental constant of nature. This statement has been usedsuccessfully in the past in fulfillment of the requirements forcertification and traceability. Contents Retrieved from Microscopy Listserver ArchivesHello all,I am attaching CP dried salmon skins (approx.

2-3 mm thick) to stubs withElectrodag 502 for secondary electron imaging. The adhesive is a graphitepaint in MEK base (a substitute for TV Tube Koat).

After ethanoldehydration and CP drying in liquid CO2, the samples are relatively flat orslightly curved. However, once attached to the stub the skins develop anoticeable curl which leaves a gap between the stub surface and sample. Insome cases I can see the 'trail' in the adhesive left by the skin as it wascurling! Filling the gap with more Electrodag is a waste of time becauseit evaporates and shrinks back away from the sample (thankfully it doesn'tseem to produce more curling).Could anyone suggest a better conductive adhesive?

Also, is there anydifference between aqueous (eg. Aquadag colloidal graphite) and MEK-basedmedia as to its effect on biological samples?As an SEM novice any advice you could share with me is greatly appreciated.Thank you very much,Carla AiwohiWestern Fisheries Research CenterSeattle, WAph: (206) 526-6282 ext.242fax: (206) 526-6654From daemon Thu Oct 5 10. Contents Retrieved from Microscopy Listserver ArchivesAnyone know of a easy, simple way to produce nice powder diffraction samples forillustrating the powder diffraction method for EM students?

We use theInternational Centre for Diffraction Data For student identification of unknownsamples. I have tried several methods that usually result in non-electrontransparent samples (our TEM KV max is only 120). Any ideas?Dear Bill,The simplest way I know is to evaporate a layer of Au or Al onto aformvar-covered grid. By spreadingthe beam, you can get continuous rings, and by using a small selected area youcan get discrete spots at various positions around the rings, so you candemonstrate directly that the rings consist of the summed intensities from manycrystalites of differing orientations.

Good luck.Yours,Bill TivolWadsworth CenterAlbany NY(518) 473-7399 WFT02-at-health.state.ny.usFrom daemon Thu Oct 5 10. Contents Retrieved from Microscopy Listserver ArchivesSimon,You could prepare your own. I find that the diatoms in diatomaceous earth areusually broken and like to prepare my own. You will need a plankton net, somepotassium dichromate, and 30% peroxide.

Once the diatoms are collected(plankton net tows from shoreline) you can clean them using the 30% peroxideand potassium dichromate. Place the diatoms in a large beaker (and I meanlarge here) then pour in 15 to 20 mls of peroxide followed by a large pinch ofthe potasium dichromate. The resultant reaction oxidizes organics leaving theglass frustules of the diatoms unharmed. It is better to be cautious with theamounts that you use as this is an exothermic reaction, sometimes violentlyso. Not that you'll have an explosion, but I've had to clean up boil overs ona number of occasions. Once the reaction subsides and the solution turns agolden yellow color it is just a matter of washing the diatoms.

There are acouple of ways to do this. You could centrifuge the diatoms, then throw outthe liquid, add water, centrifuge, throw out the liquid, etc.

Or you could fillthe large beaker with water and let the diatoms settle to the bottom (1-2hrs),carefully pour off the fluid, and add more water, let the diatoms settle, etc.The diatoms will appear as a milky white residue in the bottom of yourcontainer (you may have to concentrate the material to really see this). Ifyou need any more info please feel free to contact me.Greg'swoodland-at-madasafish.com'-at-sparc5.microscopy.com wrote: - Email: swoodland-at-madasafish.com Name: Simon Woodland School: Kaskenmoor School Question: Where can I get some diatoms to put under our microscope? How should they be prepared and mounted.Greg StroutElectron Microscopist, University of OklahomaWWW Virtual Library for Microscopy:gstrout-at-ou.eduOpinions expressed herein are mine and not necessarily those ofthe University of OklahomaFrom daemon Thu Oct 5 10.

Contents Retrieved from Microscopy Listserver ArchivesBart and PeterBesides Nikon's own release (EU-1 wired remote control) forthe CoolPix990, you can purchase a third-party release cablefrom the CKCpower.com website.Jim Hi Peter, I think one can use the 'self timer' function of the Coolpix 990 to expose an image without touching the camera body at the time of the exposure. I can think of no engineering reason why the cable release would need to be expensive when it becomes available. I'll bet any electronics tinkerer could make one for $30. I'm quite happy with my Cool-Pix 990.

If only I could remember 10% of its function modes and how to access them. Bart Cannon Cannon Microprobe SeattleFrom daemon Thu Oct 5 11. Contents Retrieved from Microscopy Listserver ArchivesWe have a Ge detector and get considerably better resolution with it than weget with an older SiLi detector. The Ge detector has an ultrathin window,the Si detector has a Be window. They are both used for x-ray detection inthe 0-20 KeV range.Kent Ross-Kent RossResearch AssociateTexas Center for SuperconductivityUniversity of HoustonDKROSS-at-uh.edu713-743-8284-From daemon Thu Oct 5 11. Contents Retrieved from Microscopy Listserver ArchivesBrian-You don't mention at what magnifications you see the sawtooth.

However, ifyou see it in the alphanumerics, I can't see that it can be anything otherthan a problem in your display unit - a 60Hz interference in the line scandrive (but not the scan generator) or something like that. If that is thecase, the magnification wouldn't make any difference.Good luck,Tony Garratt-Reed hi all- i've installed a 35 and have some issues with a saw-tooth distortion in the scan. It stays constant regardless of KV, but the number of saw-tooths (teeth?) in a frame increases with slower scan rates. I suspected vibration so i mounted the roughing pumps on a big foam block.no change. I also cycled off the chiller unit with no change, so its probably not mechanical vibrations, unless there is another vibration causing component i'm overlooking. the distortion is also visible in the data field alphanumerics (they shift and shimmy a little) with the beam turned off, so i don't suspect a HV instability problem. can i logically deduce that there is an electrical field leakage that is messing up the scan?

Or a ground loop? What about cable placement? Or a problem with the 100V variac (its within 3' of the column). right now the column is a bit dirty and needs cleaning.i've never seen, for example, dirty apertures cause this type of distortion though. any suggestions would be appreciated! brian - Brian McIntyre Electron Microscopy Lab- River Campus Univ of Rochester Rochester, NY 14627 716-275-3058/4875.

Anthony J. Garratt-Reed. MIT Room # 13-1027. 77 Massachusetts Avenue. Cambridge, MA.

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USA. Phone: (+) 1-617-253-4622. Fax: (+) 1-617-258-6478.From daemon Thu Oct 5 13. Contents Retrieved from Microscopy Listserver ArchivesWe currently have 4 Ge EDX detectors and 1 Si(Li) detector, all from OxfordInstruments.

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The Ge detectors do have a lower background than the Si(Li) andthe resolution is better. The Ge detectors range from 109 ev to 115 evwhereas the Si(Li) is around 132 ev. We routinely use all of them for low-kVwork. The Ge detectors do a better job because of the resolution and thelower background. I do SEM of semiconductors, so 30kV is as high as I cango. Contents Retrieved from Microscopy Listserver ArchivesCarolina Biological Supply has diatoms for this purpose.I just take them out of the jar with a dropper and make a wet mount with acoverslip.You can get them live or preserved.www.carolina.comMatthew J.